Genome analyses of the wheat yellow (stripe) rust pathogen Puccinia striiformis f. sp. triticireveal polymorphic and haustorial expressed secreted proteins as candidate effectors

Table 4 Secreted proteins with non-synonymous substitutions between UK isolates PST-87/7 and PST-08/21 and in tribes ranking within the top 100 potential effector tribes

Gene ID	Tribe no.	Tribe ranking	Length (amino acids)	Similarity to HESPs or fungal AVRs	1 FIR ≥10 Kb	No. of repeat units	SCR protein	Effector motifs (amino acid position)	NLS signal	PFAM mapping	Infected material (reads)	Haust. 1 (reads)	Haust. 2 (reads)	Enrichment in haustoria
														Fold change (log2)	Pvalue (adj)
PST130_14637	9	6	609	No	No	10	No	-	No	No	0	0	4	1.02	0.68
PST21_19014	11	14	167	No	No	0	No	Y/F/WxC(85); LIAR(32)	No	Yes	535	120	534	-0.38	0.87
PST887_17743	11	14	185	No	No	0	No	Y/F/WxC(103); LIAR(32)	No	Yes	904	167	695	-0.61	0.70
PST130_00418	8	17	176	No	No	20	No	-	No	No	6	0	361	4.15	1.00
PST21_12116	74	20	455	No	No	0	No	-	No	Yes	298	3708^b	34272^b	5.99	<0.001
PST130_05023	351	22	281	No	No	6	No	-	No	Yes	2475^a	7368^b	62158^c	3.85	0.011
PST21_18360	437	23	394	No	No	0	No	-	No	No	480	5957^b	15413^b	5.08	0.001
PST130_00285	317	25	207	No	No	0	No	-	No	Yes	919	6699^b	36240^b	4.77	0.001
PST21_15642	308	28	102	No	No	0	Yes	-	No	No	209	6002^b	6286^a	5.96	<0.001
PST21_17946	17	39	177	No	No	3	No	-	No	No	396	417	3015	2.21	0.19
PST21_04206	456	58	515	No	No	0	No	-	No	Yes	2563^a	513	1806	-0.70	0.65
PST21_20471	21	83	376	No	No	13	No	-	No	No	24	7	34	0.05	0.69
PST43_15488	111	94	94	No	No	0	Yes	Y/F/WxC(23)	No	Yes	399	0	76	-3.81	0.17
PST21_15274	111	94	91	No	No	0	Yes	Y/F/WxC(23)	No	No	64	1	24	-2.46	0.17

HESPs, Haustorial expressed secreted proteins. FIR, flanking intergenic region. SCR, small cysteine-rich. NLS, nuclear-localization signal. Haust, Haustorial library.
Genes expressed in the top 10 (a), 5 (b) and 1% (c) in a particular RNAseq dataset.

ISSN: 1471-2164