Figure 4

Phylogenetic relationships among completely sequenced Pseudomonas species. Phylogenetic tree for members of the genus Pseudomonas was constructed based on aligned concatenated sequences of gyrB and rpoD using the neighbor-joining method with 1000 bootstrap replicates. Analysis was carried out using Phylip 3.67 software and the tree was plotted using iTOL software. Colors on the phylogenetic tree indicate membership in Pseudomonas phylogenetic groups according to NCBI taxonomy. Completely sequenced species in the genus Pseudomonas include P. aeruginosa (yellow), P. brassicacearum (olive), P. entomophila (purple), P. fluorescens (green), P. fulva (blue), P. mendocina (pink), P. putida (navy), P. stutzeri (magenta), and P. syringae (cyan). In this research, the tree branch of P. chlororaphis, whose draft genome sequence was reported recently, is shown in red. Bar chart associated with nodes indicates numbers of genes conserved between GP72 and the corresponding organism. Conserved genes were determined using mGenomeSubtractor.