Screening of differentially expressed genes in the growth plate of broiler chickens with Tibial Dyschondroplasia by microarray analysis

Table 2 Primers used for qPCR analysis

Gene	Gene descriptions	Primers sequence (5′-3′)	Target size (bp)	Tm (°C)
lox	lysyl oxidase	Forward: TACGTGCAGAGGATGTCCATGT	114	60
lox	lysyl oxidase	Reverse: TCTCAGGAGCACTCGGTTGTC	114	60
hsp25	heat shock protein 25	Forward: AGGAGTGTGCCCATCCAGGT	109	60
hsp25	heat shock protein 25	Reverse: GATGCAGACCGTTGTTCCGT	109	60
id1	inhibitor of DNA binding 1	Forward: GGGGTCATTGCCGACATT A	115	60
id1	inhibitor of DNA binding 1	Reverse: AGGGGGGACTCAGGAATGTA	115	60
ktn1	kinectin 1	Forward: CGGTGAATCTTAACCAGGATGTAG	134	60
ktn1	kinectin 1	Reverse: TGCTATCCTCAGATCAGCGATT	134	60
sfrp4	secreted frizzled-related protein 4	Forward: GCTGAATCTATCTGCTGTTGGG	136	60
sfrp4	secreted frizzled-related protein 4	Reverse: CTAACACTGTAAGCATATTTCTGGC	136	60
cdh1	cadherin 1	Forward:TGAGAAGCAGATACTGAGCATTGTG	122	60
cdh1	cadherin 1	Reverse: GCTCATCTTGGCCCCTTATCTC	122	60
eno2	enolase 2	Forward: GTGTGCGTGTGTGTAGGTGTATGT	113	60
eno2	enolase 2	Reverse: AGTGCTCAGAACGGAAGGAAGA	113	60
rps16	ribosomal protein S16	Forward: ACAAACTGCTTGAACCTGTCCTC	127	60
		Reverse: GCTTTGGAAATAGCTTGACGG

ISSN: 1471-2164