Figure 4

Anthocyanin profiles of red petals of genotypes segregating for this phenotype. Analysis of anthocyanins in red Actinidia petals by Ultra High Performance Liquid Chromatography (UHPLC)(WVL 530 nm) identified four patterns that commonly occurred. Traces 1 to 4 show the anthocyanins present, and their concentrations, in the sample as: a - delphinidin 3-O-(xylosyl)galactoside; b - delphinidin 3-O-galactoside; c – cyanidin 3-O-(xylosyl)galactoside; d - cyanidin 3-O-galactoside; e – cyanidin 3-O-glucoside. Trace 1 is dominated by cyanidin 3-O-galactoside/cyanidin 3-O-glucoside. Cyanidin 3-O-(xylosyl)galactoside is the most plentiful anthocyanin present in trace 2, while trace 3 shows a significant concentration of delphinidin 3-O-galactoside together with cyanidin 3-O-galactoside/cyanidin 3-O-glucoside. Trace 4 shows a pattern of multiple anthocyanins with delphinidin 3-O-(xylosyl)galactoside, delphinidin 3-O-galactoside, cyanidin 3-O-(xylosyl)galactoside, cyanidin 3-O-galactoside and cyanidin 3-O-glucoside all present.