BMC Genomics

Table 2 Allelic composition of the parents and progeny of the F ₂ hybrid backcross

From: An R2R3 MYB transcription factor determines red petal colour in an Actinidia(kiwifruit) hybrid population

Progeny sample	G	Fam	Petal colour	Ke923 (MYB110a) alleles		Ke701 (MYB110b) alleles
				1	2	1	2	3	4	5	6	7	8	9	10
21-05-08g	m	1	red	209	228		361						426
21-05-08j	m	1	red	209	228		361						426
21-07-09c	f	1	red	209	228		361						426
21-06-10h	f	1	red	209	228		361							427
21-07-09f	m	1	red	209	228		361							427
21-07-09g	f	1	red	209	228		361							427
21-07-09b	f	1	white		228						410		426
21-07-12e	m	1	white		228						410		426
21-08-08h	f	1	white		228						410		426
21-07-09a	m	1	white		228						410			427
21-07-12b	f	1	white		228						410			427
21-08-08b	f	1	white		228						410			427
21-05-09f	m	2	red	209	228	359							426
21-07-13d	m	2	red	209	228	359							426
21-06-11i	f	2	red	209	228	359								427
21-07-13e	f	2	red	209	228	359								427
21-08-11a	m	2	red	209	228	359								427
21-05-09b	m	2	white		228								426		432
21-05-09e	f	2	white		228								426		432
21-05-09h	m	2	white		228								426		432
21-05-09g	f	2	white		228									427	432
21-07-13g	f	2	white		228									427	432
21-07-14g	m	2	white		228									427	432
21-05-12i	f	3	red	209	228	359							426
21-07-11c	m	3	red	209	228	359							426
21-09-10c	f	3	red	209	228	359							426
21-05-12a	f	3	red	209	228	359								427
21-05-12g	f	3	red	209	228	359								427
21-07-11b	m	3	red	209	228	359								427
21-06-13a	m	3	white		228								426
21-08-09h	f	3	white		228									427
21-06-08a	f	4	red	209	228	359							426
21-06-12b	m	4	red	209	228	359							426
21-07-08h	f	4	red	209	228	359							426
21-07-10b	m	4	red	209	228	359							426
21-09-11f	m	4	red	209	228	359							426
21-06-08h	f	4	red	209	228	359								427
21-07-10h	f	4	red	209	228	359								427
21-09-09a	f	4	red	209	228	359								427
21-09-09d	m	4	red	209	228	359								427
21-07-08a	m	4	white		228								426
21-07-08b	f	4	white		228								426
21-07-10e	f	4	white		228								426
21-07-10f	m	4	white		228								426
Parents
CK01_03	f		white	nd							410	413
CK01F2	f		white		228			406	408
CK15_01	m		white		228								426
CK15_02	m		white		228								426	427
CK21_01	f		white		228					409					432
EA03_01	f		red	209		359	361
EA04_03	f		red	209	228	359
EA07_R22	m		red	209	228	nd
Hort16A	f		white		228			406					426
11-06-17c	f	1	red	209	228		361				410
11-06-16e	f	2	red	209	228	359									432
11-06-15d	f	3	red	209	228	359							426
11-06-15c	f	4	red	209	228	359							426

Analysis by capillary electrophoresis of PCR products with the MYB110 gene markers showed that both MYB110a and MYB110b carried alleles that segregated with red petal colour. In all the parents available for analysis, and progeny, only two alleles were amplified with MYB110a marker primers. Red-petalled Actinidia genotypes carried alleles represented by PCR products sized 209 (allele 209) and 228 (allele 228), while white-petalled genotypes were homozygous for allele 228. Analysis with MYB110b showed a total of ten alleles amplified in parents. Of these ten alleles, only six were found in the F₂ backcross progeny. Two of the six alleles, alleles 359 and 361, segregated with red petal colour. The presence of either allele gave the red phenotype.

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ISSN: 1471-2164

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