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Table 2 Allelic composition of the parents and progeny of the F 2 hybrid backcross

From: An R2R3 MYB transcription factor determines red petal colour in an Actinidia(kiwifruit) hybrid population

Progeny sample G Fam Petal colour Ke923 (MYB110a) alleles Ke701 (MYB110b) alleles         
     1 2 1 2 3 4 5 6 7 8 9 10
21-05-08g m 1 red 209 228   361       426   
21-05-08j m 1 red 209 228   361       426   
21-07-09c f 1 red 209 228   361       426   
21-06-10h f 1 red 209 228   361        427  
21-07-09f m 1 red 209 228   361        427  
21-07-09g f 1 red 209 228   361        427  
21-07-09b f 1 white   228       410   426   
21-07-12e m 1 white   228       410   426   
21-08-08h f 1 white   228       410   426   
21-07-09a m 1 white   228       410    427  
21-07-12b f 1 white   228       410    427  
21-08-08b f 1 white   228       410    427  
21-05-09f m 2 red 209 228 359        426   
21-07-13d m 2 red 209 228 359        426   
21-06-11i f 2 red 209 228 359         427  
21-07-13e f 2 red 209 228 359         427  
21-08-11a m 2 red 209 228 359         427  
21-05-09b m 2 white   228         426   432
21-05-09e f 2 white   228         426   432
21-05-09h m 2 white   228         426   432
21-05-09g f 2 white   228          427 432
21-07-13g f 2 white   228          427 432
21-07-14g m 2 white   228          427 432
21-05-12i f 3 red 209 228 359        426   
21-07-11c m 3 red 209 228 359        426   
21-09-10c f 3 red 209 228 359        426   
21-05-12a f 3 red 209 228 359         427  
21-05-12g f 3 red 209 228 359         427  
21-07-11b m 3 red 209 228 359         427  
21-06-13a m 3 white   228         426   
21-08-09h f 3 white   228          427  
21-06-08a f 4 red 209 228 359        426   
21-06-12b m 4 red 209 228 359        426   
21-07-08h f 4 red 209 228 359        426   
21-07-10b m 4 red 209 228 359        426   
21-09-11f m 4 red 209 228 359        426   
21-06-08h f 4 red 209 228 359         427  
21-07-10h f 4 red 209 228 359         427  
21-09-09a f 4 red 209 228 359         427  
21-09-09d m 4 red 209 228 359         427  
21-07-08a m 4 white   228         426   
21-07-08b f 4 white   228         426   
21-07-10e f 4 white   228         426   
21-07-10f m 4 white   228         426   
Parents                
CK01_03 f   white nd        410 413    
CK01F2 f   white   228    406 408       
CK15_01 m   white   228         426   
CK15_02 m   white   228         426 427  
CK21_01 f   white   228      409      432
EA03_01 f   red 209   359 361         
EA04_03 f   red 209 228 359          
EA07_R22 m   red 209 228 nd          
Hort16A f   white   228    406      426   
11-06-17c f 1 red 209 228   361     410     
11-06-16e f 2 red 209 228 359          432
11-06-15d f 3 red 209 228 359        426   
11-06-15c f 4 red 209 228 359        426   
  1. Analysis by capillary electrophoresis of PCR products with the MYB110 gene markers showed that both MYB110a and MYB110b carried alleles that segregated with red petal colour. In all the parents available for analysis, and progeny, only two alleles were amplified with MYB110a marker primers. Red-petalled Actinidia genotypes carried alleles represented by PCR products sized 209 (allele 209) and 228 (allele 228), while white-petalled genotypes were homozygous for allele 228. Analysis with MYB110b showed a total of ten alleles amplified in parents. Of these ten alleles, only six were found in the F2 backcross progeny. Two of the six alleles, alleles 359 and 361, segregated with red petal colour. The presence of either allele gave the red phenotype.