Gene Ontology total transcriptome. The Gene Ontologies (GO) of succesfully annotated genes in the total transcriptome were determined by BLAST2GO and GO terms were subsequently condensed using the generic GO Slim subset. The histogram details the number of Pararge aegeria contigs (note, for some genes multiple contigs were present in the transcriptome) for each GO term. FPKM estimates were used to compare the levels of transcripts found in the ovaries and as maternal transcripts in the egg. Using a Log2 fold change threshold of 1, genes were classified in the histogram as present in similar amounts in the egg and ovarian transcriptome (labelled Ubiquitous), used predominantly during oogenesis to make an egg, but not or hardly used as a maternal transcript (labelled Ovary), or highly concentrated in the egg as maternal transcripts (labelled Egg).