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Table 2 List of genes identified in this study that are influenced by PrfA, σB, or VirR, are required for epithelial cell invasion or intracellular replication, are induced during intracellular replication, or are homologs of internalin

From: Acid shock of Listeria monocytogenes at low environmental temperatures induces prfA, epithelial cell invasion, and lethality towards Caenorhabditis elegans

Locus

Gene

Functional annotation or prediction

Comments from literature

T

min after acid shock

Adaptation1

     

15

30

60

120

 

Induced after acid shock at both 25°C and 37°C

lmo0206

 

Hypothetical protein

PrfA-influenced, up regulated intracellularly [38]

25°C

1*

2.0

3.2

3.1

0.9

37°C

1.4

4.1

2.4

1*

1*

lmo0223

cysK

CysK, highly similar to cysteine synthase

PrfA-box upstream, cell wall proteome [47]

25°C

2.3

6.2

6.6

4.1

n.a.

37°C

2.5

3.9

2.5

2.9

2.0

lmo0593

 

Nitrate transporter

Up regulated intracellularly [38]

25°C

0.6

1*

1.9

2.2

0.4

37°C

6.2

5.7

6.6

2.9

1*

lmo0596

 

Hypothetical protein

Contains PrfA- and RpoS-boxes [48], σB-dependent [35]

25°C

0.8

1.7

2.0

1.7

0.5

37°C

10.1

5.9

4.6

3.3

5.7

lmo0610

inl

Peptidoglycan bound protein (LPXTG motif)

Internalin-homolog [49]

25°C

1.5

3.7

6.4

5.7

0.4

37°C

9.1

5.9

5.4

1*

1*

lmo0669

 

Oxidoreductase

σB[50] and PrfA-regulated [48], required for intracellular survival [51]

25°C

1.8

4.4

8.3

7.7

0.7

37°C

3.6

5.7

5.0

3.7

3.8

lmo0759

 

Lactoylglutathione lyase

Mutant is intracellularly attenuated [39]

25°C

2.6

4.9

8.2

11.0

0.8

37°C

4.8

4.9

4.5

2.9

1*

lmo0783

 

Mannose-specific PTS component IIB

σB-dependent [52]

25°C

0.7

1*

2.2

2.0

0.8

lmo0880

 

Cell wall associated protein precursor (LPXTG motif)

Up regulated intracellularly [38], σB-regulated [53]

25°C

2.4

6.9

9.1

8.8

1*

37°C

50.1

18.8

12.5

23.9

17.4

lmo1138

clpP

Proteolytic component of Clp protease

Up regulated intracellularly [38]

25°C

n.a.

1.8

3.4

4.5

n.a.

37°C

2.0

4.5

2.8

1.4

n.a.

lmo1293

glpD

Glycerol-3-phosphate dehydrogenase

Up regulated intracellularly [38]

25°C

n.a.

4.9

7.7

5.9

n.a.

   

Required for intracellular growth [39]

37°C

5.7

4.4

3.3

5.3

7.0

lmo1302

lexA

SOS response regulator

Up regulated intracellularly [38]

25°C

1.8

2.8

3.5

5.0

1*

37°C

2.0

2.4

3.5

2.2

1*

lmo1416

 

Hypothetical protein

Deletion renders L. monocytogenes bile sensitive [36]

25°C

1*

1.7

7.8

11.5

4.7

37°C

1*

8.9

7.7

9.4

5.9

lmo1734

gltS

Glutamate synthase (large subunit)

Intracellularly up regulated [39]

25°C

1.7

2.0

5.2

1*

1*

37°C

1*

6.9

6.0

1*

2.4

lmo1991

ilvA

Threonine dehydratase

Involved in intravacuolar survival [38], intracellularly up regulated [39]

25°C

1.7

2.1

2.9

3.1

0.9

37°C

2.7

3.3

3.8

2.9

1*

lmo2085

 

Peptidoglycan bound protein (LPXTG motif)

Up regulated intracellularly [38], σB-dependent [53]

25°C

1.7

6.3

9.4

7.3

0.7

37°C

6.1

5.8

3.9

4.2

1*

lmo2156

 

Hypothetical protein

VirR regulated [54]

25°C

8.3

18.0

11.1

4.6

2.0

37°C

6.2

9.9

4.6

4.6

1.7

lmo2191

spxA

Hypothetical protein

Up regulated intracellularly [38]

25°C

1*

1.9

3.6

3.6

0.8

37°C

6.2

4.7

4.1

4.1

2.8

lmo2199

ohrA

Hypothetical protein

Involved in hydroxyperoxide resistance [55], up regulated intracellularly [38]

25°C

1*

1.7

3.4

7.4

1.5

37°C

1.5

2.1

3.8

3.9

1*

lmo2200

ohrR

Transcription regulator

Involved in hydroxyperoxide resistance [55], up regulated intracellularly [38]

25°C

1*

2.5

5.2

10.2

2.0

37°C

9.8

11.2

11.2

14.0

3.4

lmo2206

clpB

similar to endopeptidase Clp ATP-binding chain B

chaperone, up regulated intracellularly [38, 39]

25°C

2.9

5.8

5.8

4.1

n.a.

37°C

7.2

6.9

3.5

1.5

n.a.

lmo2434

gadD3 /gadD

Glutamate decarboxylase

Up regulated during acid stress [15] and intracellularly [39], σB-dependent [56]

25°C

1*

5.7

10.1

11.1

0.4

37°C

1.8

2.5

2.4

1*

1.5

lmo2570

 

Integral membrane protein

PrfA-regulated [48]

25°C

0.7

2.5

5.8

7.6

0.8

37°C

2.1

4.1

3.4

2.7

1*

lmo2571

 

Nicotinamidase

PrfA-regulated [48]

25 °C

0.5

4.1

3.4

2.7

1*

37°C

12.6

3.6

8.2

10.1

1*

lmo2572

 

Dihydrofolate reductase, chain A

PrfA-regulated [48]

25°C

1*

3.9

8.6

9.1

1*

37°C

5.5

3.9

n.a.

3.7

2.3

lmo2573

 

Zinc-binding dehydrogenase

PrfA-regulated [48]

25 °C

2.3

6.8

10.6

10.0

0.5

37°C

14.3

6.1

6.2

4.3

6.5

lmo2695

 

Dihydroxyaceton kinase

Mutant attenuated in macrophages (Fuchs et al., unpublished data)

25°C

1*

5.5

8.2

5.9

0.8

37°C

2.8

2.6

2.6

2.8

2.1

lmo2696

 

Dihydroxyaceton kinase

Mutant attenuated in macrophages (Fuchs et al., unpublished data)

25°C

n.a.

5.3

13.5

18.6

n.a.

37°C

1.8

5.5

3.2

2.3

1.7

lmo2713

 

Secreted protein with GW repeat

Up regulated intracellularly [38], σB-regulated [48]

25°C

1.5

3.0

5.0

3.9

1*

37°C

3.1

2.5

4.0

2.8

1.5

Induced after acid shock at 25°C, but not at 37°C

lmo1538

glpK 1

Glycerol kinase

Up regulated intracellularly [39]

25°C

4.3

3.5

2.5

1.5

4.6

Induced after acid shock at 37°C, but not at 25°C

lmo0232

clpC

Endopeptidase Clp, ATP-binding chain C

Plays a role in adhesion and invasion, and modulates the expression of InlA, InlB and ActA [57]

37°C

2.7

9.6

6.0

2.0

1.8

lmo1439

sod

Superoxide dismutase

Mutant attenuated in survival within macrophages [58]

37°C

1*

1*

2.5

2.7

2.0

lmo2250

arpJ

ABC-transporter for arginine

Mutant attenuated in intracellular growth [59]

37°C

1*

2.3

2.0

2.0

1*

  1. See legend of Table 1.
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BMC Genomics

ISSN: 1471-2164

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