Skip to main content

Table 3 The positional relationship of primer, methylation peak and transcript start site (TSS) in genes selected for validation

From: Genome-wide promoter methylation profile of human testis and epididymis: identified from cell-free seminal DNA

Gene Gene name GeneBank accession no. Primer location(5′-3′) Peak location Strand Position(TSS = +1)
Forword Reverse Forword 5′ Reverse 5′
PRAME Preferentially expressed antigen in melanoma NM_206956 chr22:21231279 chr22:21231496 chr22:21231050 - 251 54
−21231299 −21231479 −21231499
PEG10 Paternally expressed 10 NM_015068 chr7:94124064 chr7:94124274 chr7:94123914 + 492 702
−94124086 −94124252 −94124663
MORC1 MORC family CW-type zinc finger 1 NM_014429 chr3:110319361 chr3:110319519 chr3:110319258 - 322 164
−110319381 −110319495 −110320007
GML Glycosylphosphatidylinositol anchored molecule like protein NM_002066 chr8:143913400 chr8:143913599 chr8:143912618 + 182 381
−143913419 −143913577 −143913667
HOXA5 Homeobox A5 NM_019102 chr7:27150841 chr7:27151029 chr7:27150312 - −1029 −1217
−27150862 −27151008 −27151961
DNMT3L DNA(cytosine-5-)- methyltransferase 3-like NM_175867 chr21:44506322 chr21:44506560 chr21:44506027 - 205 −33
−44506343 −44506540 −44508076
SNURF SNRPN upstream reading frame NM_005678 chr15:22751295 chr15:22751430 chr15:22751327 + 68 203
−22751314 −22751410 −22751676
MSH4 MutS homolog 4 NM_002440 chr1:76035117 chr1:76035360 chr1:76035117 + −100 143
−76035135 −76035338 −76035566
DAZ1 Deleted in azoospermia 1 NM_004081 chrY:23754371 chrY:23754582 chrY:23754045 - 256 45
−23754389 −23754560 −23754994
CLPB Caseinolytic peptidase B homolog NM_030813 chr11:71824486 chr11:71824650 chr11:71823916 - −1270 −1434
−71824504 −71824631 −71824774