Figure 3

Scatter- and boxplots comparing tissue-specific gene expression in the spring tidal phase. Scatter plots of gene expression (as Log₂[reads]) in a) female versus male tissue, b) vegetative versus male tissue, and c) vegetative versus female tissue. Each point on the plots represents a F. vesiculosus gene orthologue defined by mapping contigs to the E. siliculosus proteome using BLASTX. Only genes with ≥ 20 reads were analysed and are shown on the plots (total = 693). Points in green, red and blue indicate statistically significant over-expression in vegetative female and male tissue, respectively (Fisher’s exact tests with Benjamini-Hochberg adjustment; P < 0.05). Points in grey do not differ in expression between tissue types. Boxplots (lower panel) corresponding to each scatter plot show distributions of significant expression values as reads/orthologue for sets of sex- and vegetatively-biased genes: d) female versus male tissue, e) male versus vegetative tissue, and f) female versus vegetative tissue. MS+, FS+, and VS + = over-expressed in male, female, and vegetative spring tide libraries, respectively. The result of Kolmogorov-Smirnov tests on the distributions are summarized on each plot.