Figure 9
Detection of 5′ tRNA halves in mouse plasma and serum. Northern blot of RNAs extracted from 0.4 ml of mouse serum, serum treated with EDTA, and heparin- or EDTA-collected plasma, hybridized to a 32P 5′-end labeled oligonucleotide probe complementary to the 5′ end of tRNA-Gly-GCC. EDTA sharply lowers the abundance of 5′ tRNA halves in serum; in plasma, 5′ tRNA halves are abundant when heparin is the anticoagulant, but are nearly absent when EDTA is present. 5′ tRNA halves are similarly abundant when calcium citrate is used as the anticoagulant (not shown).