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Table 1 Strains and plasmids used in this study

From: Functional genomic analysis of bile salt resistance in Enterococcus faecium

Strain or plasmid Relevant characteristic(s) Source or reference
E . faecium    
E1162 Clinical isolate (bloodstream infection), isolated in France, 1996 [23]
ΔgltK Markerless deletion mutant of gltK gene of E1162 This study
ΔgspA Markerless deletion mutant of gspA gene of E1162 This study
ΔgltK+gltK Complementation strain of ΔgltK; ΔgltK harboring pEF25-gltK This study
ΔgspA+gspA Complementation strain of ΔgspA; ΔgspA harboring pEF25-gspA This study
E. coli strains    
DH5α E. coli host strain for routine cloning Invitrogen
EC1000 MC1000 glgB::repA; host strain for pWS3 derived vectors [24]
Plasmids    
pWS3 Gram-positive thermosensitive origin of replication; Spcr [29]
pDEL3a pWS3 carrying the 5′ and 3′ flanking regions of gene gltK for mutant construction This study
pDEL4a pWS3 carrying the 5′ and 3′ flanking regions of gspA gene cluster for mutant construction This study
pDEL3b pDEL3a with a Genr cassette which was flanked by lox66- and lox71-sites cloned between the 5′ and 3′ flanking regions This study
pDEL4b pDEL4a with a Genr cassette which was flanked by lox66- and lox71-sites cloned between the 5′ and 3′ flanking regions This study
pWS3-Cre pWS3 derivative expressing Cre in E. faecium [22]
pEF25 Shuttle plasmid pAT18 with spectinomycin resistance cassette cloned in the EcoRI site; Spcr Eryr [30]
pEF25-gltK Complementation plasmid for ΔgltK; pEF25 carrying gltK This study
pEF25-gspA Complementation plasmid for ΔgspA; pEF25 carrying gspA This study