Identification of the Hevea brasiliensisAP2/ERF superfamily by RNA sequencing

BMC Genomics

Table 1 List of plant materials and treatments used to isolate total RNA

Plant material	Treatment	Condition	Collected tissues for the library
Callus	MM medium	2 weeks	Embryogenic tissue
Embryogenic callus	EXP medium	4 weeks
Somatic embryo	DM medium	8 weeks
1-month-old in vitro plant	None	-	Leaf, root
1-year-old in vitro plant	None	-	Leaf, bark, root
	Wounding	1, 4 and 24 hours
	Ethylene	1 ppm for 4, 8 and 24 hours
	Water deficit	3 weeks
	Flood	72 hours
	Cold	24 hours
4-year-old in vitro plant	None	-	Root
3-month-old shoot from budded plant	None	-	Leaf, bark
	Wounding	1, 4 and 24 hours
	Ethylene	1 ppm for 4, 8 and 24 hours
	Methyl-jasmonate	0.3 μM for 1, 4 and 24 hours
5-year-old tree	Non-tapped	-	Latex, bark
	Tapped	Every 2 days
	Tapped, ethephon	Every 2 days, 2.5% once a month

RNA sequencing was carried out on a mix of RNAs from the same tissues of various plant materials grown under specified treatments. MM, EXP and DM media are used for callus proliferation, somatic embryogenesis induction and embryo development, respectively. All media are described in [55].

ISSN: 1471-2164