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Table 1 Summary of the read mapping in DNA sequencing

From: Bacillus subtilis genome vector-based complete manipulation and reconstruction of genomic DNA for mouse transgenesis

  BAC1   BAC2   Tg110CIBS   Tg220CISP   Tg250SB  
# reads % # reads % # reads % # reads % # reads %
Total reference length 114,183   220,003   115,372   222,532   254,658  
Mapped reads 8,341,236 91.1 8,604,424 95.9 1,028,479 2.7 1,737,127 5.2 504,510 5.8
Unmapped reads 814,824 8.9 370,790 4.1 37,166,207 97.3 31,564,383 94.8 8,227,736 94.2
Reads in pairs 8,327,110 91.0 8,594,722 95.8 960,578 2.5 1,714,062 5.2 498,152 5.7
Broken paired reads 14,126 0.2 9,702 0.1 67,901 0.2 23,065 0.1 6,358 0.1
Total number of reads 9,156,060   8,975,214   38,194,686   33,301,510   8,732,246  
Total read length (Mb) 915.6   897.5   3,819.5   3,330.2   873.2  
Fraction of reference covered 1.00   1.00   1.00   1.00   1.00  
Minimum coverage 241   125   28   22   6  
Maximum coverage 15147   5631   2211   1514   574  
Average coverage 7100.7   3812.3   851.5   752.2   191.0  
  1. Reads obtained from the Illumina Genome Analyzer IIx were analyzed and mapped to each reference sequence using CLC Genomics Workbench 5.5 (CLC Bio). The number of mapped reads in each sample was enough to evaluate mutations. Analyzed DNA samples are follows: original BAC clones of BAC1 and BAC2 and BGM clones of Tg110CIBS, Tg220CISP and Tg250SB.