GlnR binding sites identified by ChIP-seq during nitrogen limitation. Examples of peaks obtained for genes known to be GlnR-regulated. Binding sites were visualised by aligning the 160 million sequence reads to the M. smegmatis genome using IGV. The upper track indicates ChIP-seq data for GlnR immunoprecipitated DNA in nitrogen excess conditions, middle track shows the ChIP-seq data for GlnR immunoprecipitated DNA in nitrogen limiting conditions and the total DNA input control is shown in the bottom track. GlnR binding sites were identified upstream of (A) amtB , (B) glnA1 and (C) amt1.