In situ hybridization histochemistry for rat Cyp27a1 mRNA in brain 7 days after TBI. (A) Representative fields of injured parietal cortex with higher magnification of negative control sense probe in the same region of a different section (top inset) and neuronal staining with a large vascular profile (bottom inset). (B) Composite showing injured and contralateral hippocampal Cyp27a1 mRNA with ablation of neuronal staining, as well as heavier non-neuronal staining ipsilateral to injury. Some intense white matter staining is visible medially in the corpus callosum and the ipsilateral hippocampal fimbria (arrows). See Figure 1 legend for methodologic details.