Figure 2

AQR and PQR migration in wild type and mab-5 mutants. Micrographs of L4 animals expressing gcy-32::cfp (lqIs58) in AQR, PQR, and the URX neurons are shown. The vulva is marked by expression of egl-17::gfp (ayIs9). A) In wild type, AQR on the right migrates anteriorly to the anterior deirid region, and PQR on the left migrates posteriorly behind the anus in the phasmid ganglion. The diagram to the right indicates the expression status of mab-5 AQR and PQR and their precursors. B) In mab-5 loss of function, both AQR and PQR migrate anteriorly. C) In mab-5 gain of function, both AQR and PQR migrate posteriorly. D) Positions along the body used to score AQR and PQR migration defects in mutants in Tables 6 and 7 (see Methods).