An example motif CTC. Assume that a motif candidate is the sequence CTC. We calculated the number of hits (frequency of sequences) among all sliding windows for each DCSR. The frequency vectors of the inaccessible and accessible chromatin groups are [2, 2, 0] and [1, 0, 0], respectively. Next, we performed a paired t-test between these two frequency vectors to determine the significance of this motif in terms of differential chromatin states.