Figure 2

Phylogenetic analysis of ABCB full and half transporters of five metazoan species: (A) ABCB full transporters, (B) ABCB half transporters. Full-length ABC proteins were aligned using MUSCLE [122] and subjected to a maximum likelihood analysis using Treefinder [124]. The resulting tree was midpoint rooted. For amino acid alignment, amino acid substitution model and likelihood score of the constructed phylogenetic tree see Additional file 9 and Additional file 10. Numbers at the branch point of each node represent the bootstrap value resulting from 1000 pseudoreplicates (LR-ELW). Species, abbreviations, and color codes are: Ce, C. elegans (purple); h, H. sapiens (green); Dm, D. melanogaster (red); Dp, D. pulex (yellow); and tetur, T. urticae (blue). The scale bar represents 0.2 and 0.5 amino-acid substitutions per site in Figure 2A and Figure 2B, respectively. Accession numbers of metazoan ABC protein sequences can be found in Additional file 11 while T. urticae ABC protein sequences can be found in Additional file 12.