Figure 1

Experimental design overview. Library preparation treatments were done at varying levels of replication, as indicated by the numbers (1 to 3) next to each treatment. The number of amplification cycles (see y axis) includes those necessary to generate enough DNA for library preparation, but does not include the emPCR (454, Ion Torrent) or bridge (Illumina) amplification cycles used to build large enough populations of reads for nucleotide sequencing signal detection.