Alterations in expression of endometrial genes coding for proteins secreted into the uterine lumen during conceptus elongation in cattle

Table 2 Primer information used for quantitative real time PCR analysis of candidate genes

Entrez gene symbol	gene name	Accession number	Forward primer sequence	Reverse primer sequence	Product length (bp)
ACTB	Actin, beta	NM_173979.3	CGCCATGGATGATGATATTGC	AAGCCGGCCTTGCACAT	66
APOA1	Apolipoprotein A-I	NM_174242.3	GCTGGCCATTGAGGTCACCCAC	GCTGCCAGAAATGCCGAGCCT	103
ARSA	Arylsulfatase A	NM_001075205.1	GCCTTTGCCCGCGACCTCAT	TGGGTGTGGTGGGAGGCGTA	80
CYR61	Cysteine-rich, angiogenic inducer, 61	NM_001034340.2	TGCAGAGCTCAGTCGGAGGGC	GGCGCCGTCGATACATGTGC	111
DCN	Decorin	NM_173906.4	ACTCTTCAGGAGCTGCGTGTCCA	GCGGGTTGGTGCCAAGTTCTACG	106
LCAT	Lecithin-cholesterol acyltransferase	NM_001046069.2	CGGCCCGTCATCCTCGTGCC	AAGTCCTCCGTCTTGCGGTAGCA	104
MGP	Matrix Gla protein	NM_174707.2	GCAAAAGCCCAAGAGAGAATCCGA	ACACCATGGCATAGCGTTCGCA	103
MUC13	Mucin 13, cell surface associated	XM_002702427.2	ACGGGCTGGTGAGACCAAAACC	GCAGTCAGCTGTCCCGTTGC	116
NCDN	Neurochondrin	NM_001045931.1	TCTCCAGCTCTGCAGGGGACG	GGCCACGTGGGATTGCGACA	81
NMN	Neuromedin N	NM_173945.4	GAGCCCCCTTCAGCCTGTTCC	GCCAGAAGAATCATGCACACCAGC	104
NPNT	Nephronectin	XM_591031.5	TCTCAGCAGCCAAAGGCCCG	CTGACAGGCACAGGTCCCCT	90
NXPH3	Neurexophilin 3	NM_001192824.1	ATGACGGTCCGCCAGGCTCA	CCGCTTTCGAGGCATCCGGG	80
PENK	Proenkephalin	NM_174141.2	GACGCCGAGGACCGCGAGAG	CCATGGGGTTGCCGCTGTTCGG	120
PLIN2	Periliphin 2	NM_173980.2	TCTTCGCGCTTGGGCGTCTG	CCACCCTGGTCACCACACTCAGTT	101
RPL19	Ribosomal protein L19	NM_001040516.1	GAAAGGCAGGCATATGGGTA	TCATCCTCCTCATCCAGGTT	86
TINAGL1	Tubulointerstitial nephritis antigen-like 1	XM_003585097.1	CTCGGGAGGCCGGAGCGATA	GTCAGGCAGCGTCTCCTCGC	81

All primers were used at a concentration of 300 nM in a final reaction volume of 15 μl. A dissociation curve was included to ensure specificity of each primer pair.

ISSN: 1471-2164