Table 2 Details of the SNVs studied for DAZ , GOLY , BPY2 and TTTY4 genes
From: Fate of the human Y chromosome linked genes and loci in prostate cancer cell lines DU145 and LNCaP
Target | SNV | Oligos used | Accession no. | Product size | Enzyme for digestion | Restriction site | Fragments | Alleles | Present in copies | DU145 | LNCaP |
|---|---|---|---|---|---|---|---|---|---|---|---|
DAZ-SNV_I | SA 770 CACAGGCACTCAGTAACTATCTC | G73167 | 709 | Fsp1 | TGC/GCA | 709 | A | 1,2,3 4 | A&B | A&B | |
398 + 311 | B | ||||||||||
SA771CAGTGTTTCACCCACCACTTCTGGGT | |||||||||||
DAZ-SNV_II | SA 446 GACATCCACGTCATTAACAAACG | G73166 | 182 | Mbo1 | /GATC | 182 | A | 1 2,3,4 | A&B | A&B | |
122 + 60 | B | ||||||||||
SA 447 GGAAGCTGCTTTGGTAGATAC | |||||||||||
DAZ-SNV_III(sY586) | SA 772 GTGTGGCACATATGCCTATAAA | G63907 | 301 | Taq1 | T/CGA | 301 | A | 2 1,3,4 | A&B | A&B | |
184 + 117 | B | ||||||||||
SA 773 TTGGTACATCCAGATGCAGAT | |||||||||||
DAZ gene | DAZ-SNV_IV | SA 774 CTTCCTCATCTTTCTTGACTT | G73168 | 630 | AluI | AG/CT | 630 | A | 2 1,3,4 | A&B | A&B |
398 + 262 | B | ||||||||||
SA 775 TTATTTATTCCTCAAAAAGGTG | |||||||||||
DAZ-SNV_V (sY587) | SA 444 TGGTTAATAAAGGGAAGGTGTTTT | G63908 | 244 | DraI | TTT/AAA | 195 + 49 | A | 3,4 1,2 | A&B | A&B | |
122 + 73 + 49 | B | ||||||||||
SA 445 TCTCCAGGACAGGAAAATCC | |||||||||||
DAZ-SNV_VI | SA 776 GGGCCTAGTCTCTAGATCATT | G73169 | 431 | AflIII | A/CRYGT | 431 | A | 1,2,3 4 | A&B | A | |
248 + 183 | B | ||||||||||
SA 777 GCTAGAACCAAATATTCTGGAT | |||||||||||
DAZ-SNV_VII (sY581) | SA 442 CACTGCCCTAATCCTAGCACA | G63906 | 252 | Sau3AI | /GATC | 189 + 63 | A | 1,4 2,3 | A&B | A&B | |
130 + 63 + 59 | B | ||||||||||
SA 443 TCTTCTGGACATCCACGTCA | |||||||||||
GOLY1 | GOLY-SNV_1 | SA768 TTGGCCTGTTGCTTCTAGGGTT | BV012733 | 531 | HhaI | GCG/C | 531 | A | 1 Copy | A&B | A&B |
SA769 ACAGGGAGGGTGCTGTCACA | 289 + 282 | B | 1 Copy | ||||||||
BPY2 | BPY2 | SA766 AAGCCCATTGCTGAGATACTG | BV012732 | 470 | EcoRV | GAT/ATC | 470 | A | 2 Copy | A&B | A |
SA767 TTGTGATTCTGACCCAACGA | 289 + 181 | B | 1 Copy | ||||||||
TTTY4 | TTTY4 | SA764 TGCAGACAGCACTGTGGCTT | BV012731 | 541 | HaeIII | GG/CC | 541 | A | 1 Copy | A&B | A&B |
323 + 218 | B | 2 Copy | |||||||||
SA765 GTATATGGCATAATTTCACCTG |