Genomic and physiological variability within Group II (non-proteolytic) Clostridium botulinum

BMC Genomics

Table 2 Percentage of CDSs from chromosome of Eklund 17B (NRP) scored positive by microarray analysis of strains of Group II C. botulinum

Strain	Toxin subtype	Clade	Total number of CDSs present	% of control CDSs present
Eklund 17B (NRP)	B4	1	3384	100
Eklund 2B	B4	1	3384	100
IFR 05/020	B4	1	3383	100
Colworth BL151	B4	1	3383	100
IFR 05/029	B4	2	2959	87
IFR 06/001	F6	2	2917	86
IFR 05/025	B4	2	2900	86
Eklund 202F	F6	2	2863	85
2129B	B4	2	2848	84
CDC 5900	B4	2	2846	84
ATCC 17844	B4	2	2845	84
Hobbs FT50	B4	2	2829	84
Kapchunka B2	B4	2	2806	83
CDC 4627 U-1	B4	2	2792	83
Kapchunka B5	B4	2	2777	82
Hobbs FT10	F6	2	2761	82
Craig 610	F6	2	2758	82
IFR 06/005	F6	2	2750	81
CDC 3875	B4	2	2716	80
CB-K-37E	E1	3	2428	72
CB-K-32E	E6	3	2424	72
CB-K-36E	E6	3	2404	71
CB-K-24E	E1	3	2403	71
IFR 02/050	E3	3	2403	71
CDC 8073	E2	3	2401	71
CB-K-23E	E1	3	2351	69
Foster B96	E3	3	2292	68
CB-K-16E	E1	3	2281	67
CB-S-3E	E1	3	2276	67
CB-K-40E	E3	3	2263	67
CB-S-27E	NT^E	3	2249	66
CB-K-18E	E1	3	2242	66
Prevot P34	E3	3	2164	64
CB-K-34E	E1	3	2120	63
CB-S-20E	NT^E	3	2095	62
CB-S-15E	NT^E	3	2072	61
Dolman VH	E3	3	2064	61
Hazen 36208	E1	3	2058	61
CB-K-9E	E6	3	2024	60
CB-K-3E	E3	3	2023	60
CDC 7854	E1	3	2012	59
Beluga	E1	3	1990	59
CB-K-19E	E1	3	1946	58

Sixty-mer probes were used to represent 3384 chromosomal sequences of Eklund 17B (NRP) on a microarray. Data show the number of these CDSs found in each test strain by microarray analysis. CDSs were considered to be present if the test probe signal was within 8-fold of the control probe signal and significantly above the background. NT^E= no toxin gene detected but strain originally isolated containing type E neurotoxin gene.

ISSN: 1471-2164