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Table 3 Degenerate primers used to amplify R -gene fragments

From: Frequent loss of lineages and deficient duplications accounted for low copy number of disease resistance genes in Cucurbitaceae

Primer namea

Primer position (motif, domain)

Sequence 5’→3’b

15912

GGVGKTT, Kinase-1a

GGT GGG GTT GGG AAG ACA ACG

15914

GGVGKTT, Kinase-1a

GGI GGI GTI GGI AAI ACI AC

16410

GGLGKTT, Kinase-1a

GGI GGI YTI GGI AAR ACI AC

16403

GGMGKTT, Kinase-1a

GGI GGI ATI GGI AAA ACI AC

16409

GGSGKTT, Kinase-1a

GGI GGI WSI GGI AAR ACI AC

PLP

GG(G/O/M)GKTT, Kinase-1a

GGI GGI RTI GGI AAR ACI AC

310

VLDDVW, Kinase-2

CCA IAC RTC RTC NAR NAC

antiK2

VLDDVW, Kinase-2

CCA NAC RTC RTC IAR IAC

antiHD1

CKGLPL, HD

ARN GGI ARI CCY TTR CA

17609

FLDIACF, NBS-VI

RAA RCA IGC SAT RTC IAR RAA

28107

FLHIACF, NBS-IX

RAA RCA IGC DAT RTG IAR RAA

  1. a All degenerate primers used in this study were from references [41, 42].
  2. bKeys for degenerate positions: I, inosine; R,A/R; Y, C/T; K, G/T; N,A/G/C/T; A/G/T; W, A/C; S, G/C.