Figure 2

Assessment of miRNAs differentially expressed in response to AZM551248 administration. (A) Heat map generated from high-throughput sequencing data (miRNA-seq) showing miRNA expression changes in the cervical subcutaneous tissue in response to AZM551248 (n = 4 for days 4, 8, 11, 14 and 17 respectively). Data are the miRNA identifier (miRBase V16) and signed fold change relative to vehicle treated controls. Green colouration denotes significant down-regulation whilst red colouration denotes significant up-regulation. Only miRNAs were the corrected P value < 0.05 following analysis with DESeq (see materials and methods) are included within this analysis. Nomenclature: miR - mature miRNA, miR* - minor product of mature miRNA, suffix (for example a, b, c) - denotes miRNAs with closely related mature sequences. (B) Five-order Venn diagram demonstrating the number of miRNAs differentially regulated at each time point following AZM551248 administration. (C) Identification of miRNAs clustered within 10Kb of each other on the canine genome. Clusters are denoted by the inclusion of a bracket. The direction of expression change relative to vehicle treated control animals is denoted by the text colour (green down-regulated; red - up-regulated). Note the absence of any clustered miRNAs following 8 days administration of AZM551248.