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Figure 3 | BMC Genomics

Figure 3

From: Developmentally regulated expression and complex processing of barley pri-microRNAs

Figure 3

Schematic representation of the MIR166n gene and its precursors. Detection of pri-, pre- and mature miR166n. (A) MIR166n gene structure. (B) pre-miRNA166n hairpin structure (ΔG=−61 kcal/mol) and its rice orthologue (ΔG=−52.3 kcal/mol); blue and red lines indicate hybridization regions as described in Figure 1. (C) pri-miRNA166n structures (upper panel); RT-PCR analysis of their expression in five barley developmental stages studied (lower panel). (D) Real-time PCR measurements of total pri-miRNA166n expression level (upper graph) and its spliced (ΔIVS) and unspliced variants (+IVS) (lower graph); bars on the charts represent standard deviation. Values are shown as the mean ±SD (n=3) from three independent experiments. (E) Nucleotide sequence of the mature miR166n molecule, and detection of pre-miRNA166n long (L) and short (S) intermediates, and mature miR166n using Northern hybridization. U6 was used as a loading control. The levels of pre-miRNAs and miRNA were calculated as described in Figure 1. Colors, abbreviations, and symbols as in Figure 1.

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