Skip to main content
Figure 7 | BMC Genomics

Figure 7

From: Developmentally regulated expression and complex processing of barley pri-microRNAs

Figure 7

Schematic representation of the MIR1126 gene and its precursors. Detection of pri-, pre- and mature miR1126. (A) MIR1126 gene structure. (B) pre-miRNA1126 hairpin structure (ΔG=−78.4 kcal/mol) and its wheat orthologue (ΔG=−73.27 kcal/mol); blue and red lines indicate hybridization regions as described in Figure 1. (C) Structures of splice isoforms (I–V) of the miR1126 transcript; dashed lines represents unamplified 5′ fragments of the noncoding RNA isoforms IV and V; …polyA indicates a putative polyA site in splice isoforms as the determination of an accurate polyA site for PCR products is not possible. (D) RT-PCR expression analysis of splice isoforms (I–V) of the miR1126 transcript in all barley developmental stages studied. Half-open arrows on agarose gel indicate specific, identified products. (E) Real-time PCR measurements of total pri-miRNA1126 expression levels (upper graph) and pri-miR1126 fragments carrying the third intron (+IVS3) and after the third intron splicing (ΔIVS3) (lower graph); bars on the charts represent standard deviation. Values are shown as the mean ±SD (n=3) from three independent experiments. (F) Nucleotide sequence of the mature miR1126 molecule, and detection of pre-miRNA and mature miR1126 using Northern hybridization. U6 was used as a loading control. The levels of pre-miRNAs and miRNA were calculated as described in Figure 1. Colors, abbreviations, and symbols as in Figure 1.

Back to article page