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Figure 8 | BMC Genomics

Figure 8

From: Developmentally regulated expression and complex processing of barley pri-microRNAs

Figure 8

Schematic representation of the MIR1120 gene and its precursor. Detection of pri-, pre- and mature miR1120. (A) MIR1120 gene structure; black squares in the gene and pri-miRNA1120 schemes show position of the ORF. (B) pre-miRNA1120 hairpin structure (ΔG=−42.3 kcal/mol) and its wheat orthologue (ΔG=−63.5 kcal/mol); blue and red lines indicate hybridization regions as described in Figure 1. (C) pri-miRNA1120 structure and RT-PCR expression analysis in the five barley developmental stages studied. (D) Real-time PCR measurements of total pri-miRNA1120 expression levels; bars on a chart represent standard deviation. Values are shown as the mean ± SD (n=3) from three independent experiments. (E) Nucleotide sequence of the mature miR1120 molecule, and detection of pre-miRNA and mature miR1120 using Northern hybridization. U6 was used as a loading control. The level of pre-miRNAs and miRNA was calculated as described in Figure 1. Colors, abbreviations, and symbols as in Figure 1. Asterisk on agarose gel indicates unspecific product.

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