Figure 4

Variation in transcript accumulation among F1 hybrid individuals. A) Distribution of the coefficient of variation (CV) for 16,312 transcripts analyzed from F1 plants; grey = CV calculated from full data, light blue = CV calculated from reduced data (minus F1.TA), darker blue indicates overlap. B) Heatmap showing z-score normalized transcript accumulation for 166 reference contigs with CV > 2 within F1 samples. Samples from individual plants are shown in horizontal rows; F1 = hybrid F1, A = H.annuus cmsHA89, P = H. petiolaris Pet2152. Hierarchical clustering estimated from Spearman correlation coefficients for pairwise contig (x-axis) and sample (x-axis) distance matrices. The colored bar along the top edge indicates assignment of transcripts to GO Biological Process groups, with prominent categories: green (cell cycle/mitosis), yellow (histones/chromatin modification), blue (metabolism), red (stress/defense), and pink (transcription factors/signaling).