Allelic expression analysis in single bovine embryos. a) Number of SNP detected in each sample. Left bar indicates the total number of SNP detected. Right bar, represents the number of SNP that matched dbSNP variants by position. More than 99% of those matches agreed on nucleotide identity. b) Allelic bias in heterozygous SNP expression. Number of SNP expressed at different major allelic frequencies. Average number of validated (left bar) and putative (right bar) SNPs are presented separate. The number of SNP with statistically significant imbalance (FDR < 0.01 by X2 test) is indicated within their respective group (shaded area). The 85-95% frequency only includes validated SNPs.