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Table 1 Effect of end trimming on read alignment to genome

From: RNA-seq analysis of single bovine blastocysts

5′ Trim (bp) 3′ Trim (bp) % reads mapped to bovine genome (BTAU4.0)
BL 1 BL 2 BL 3 BL 4 BL 5 Mean ± SEM
0 0 67.9 70.1 70.0 67.7 70.0 69.1 ± 0.5
2 2 80.7 82.8 82.5 81.7 82.5 82.0 ± 0.4
0 4 69.7 72.6 71.9 69.7 72.0 71.2 ± 0.6
0 9 72.0 74.6 73.9 71.8 74.0 73.3 ± 0.6
4 0 83.4 84.2 85.0 84.3 84.9 84.4 ± 0.3
9 0 88.0 89.4 89.6 88.7 89.3 89.0 ± 0.3