Table 1 Effect of end trimming on read alignment to genome
5′ Trim (bp) | 3′ Trim (bp) | % reads mapped to bovine genome (BTAU4.0) | |||||
|---|---|---|---|---|---|---|---|
BL 1 | BL 2 | BL 3 | BL 4 | BL 5 | Mean ± SEM | ||
0 | 0 | 67.9 | 70.1 | 70.0 | 67.7 | 70.0 | 69.1 ± 0.5 |
2 | 2 | 80.7 | 82.8 | 82.5 | 81.7 | 82.5 | 82.0 ± 0.4 |
0 | 4 | 69.7 | 72.6 | 71.9 | 69.7 | 72.0 | 71.2 ± 0.6 |
0 | 9 | 72.0 | 74.6 | 73.9 | 71.8 | 74.0 | 73.3 ± 0.6 |
4 | 0 | 83.4 | 84.2 | 85.0 | 84.3 | 84.9 | 84.4 ± 0.3 |
9 | 0 | 88.0 | 89.4 | 89.6 | 88.7 | 89.3 | 89.0 ± 0.3 |