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Table 2 Number and proportion of reads mapped to different genome annotations and different genome builds

From: RNA-seq analysis of single bovine blastocysts

 

Annotation source and genome build

NCBI Btau 4.2

NCBI UMD 3.1

Ensembl Btau 4.0

Ensembl UMD 3.1

Number of genes in annotation

24,359

25,577

25,670

24,616

Number of transcripts in annotation

19,757

20,681

26,977

22,118

Mean number of reads processed per embryo (Million reads ± SEM)

37.6 ± 1.1

37.6 ± 1.1

37.6 ± 1.1

37.6 ± 1.1

Total number of reads mapped to annotated genes (Million reads ± SEM)

22.9 ± 0.6

24.6 ± 0.6

27.0 ± 0.7

28.9 ± 0.8

% total reads mapped

60.9%

65.6%

71.9%

76.9%

Number of uniquely mapped reads (Million reads ± SEM)

18.0 ± 0.5

18.2 ± 0.6

23.8 ± 0.7

25.5 ± 0.7

% reads uniquely mapped

48.1%

48.4%

63.2%

67.7%