Table 1 Functional grouping of genes differentially expressed (≥2-fold and P < 0.05, n = 3) between PCV2-infected and mock-infected control

Immune response

rho GDP dissociation inhibitor beta

ARHGDIB

2.16

0.41

1.21

13.11

NM_001009600

CMRF35-like molecule 1

CLM1

2.35

0.95

1.38

13.11

AY457047

cathepsin C

CTSC

2.12

3.06

1.74

11.46

NM_001814

cathepsin S

CTSS

0.5

1.09

0.77

19.25

M90696.1

Fc fragment of IgG low affinity II b receptor

FCGR2B

3.47

0.54

1.85

8.49

NM_001033013

neutrophil cytosolic factor 4

NCF4

2.62

0

2.12

6.28

NM_000631

Humoral immune response

chemokine ligand 16

CCL16

3.85

4.64

3.24

4.93

NM_004590;

CD74 molecule

CD74

3.94

0

3.43

0.58

NM_213774

complement factor D

CFD

0.36

0.41

0.59

2.88

NM_001928

C-type lectin domain family 2, member B

CLEC2B

2.83

1.62

2.10

1.09

NM_005127

Inflammatory response

chemokine ligand 3-like 3

CCL3L3

7.58

0

2.46

1.64

NM_001001437

chemokine Ligand 5

CCL5

1.53

14.04

2.25

2.44

AK312212

chemokine ligand 5

CXCL5

32.28

0

12.96

0

EU176356

chemokine ligand 7

CXCL7

2.75

0.95

3.57

1.97

NM_213862.1

interleukin-6

IL6

2.26

4.65

1.36

13.43

NM_214399.1

S100 calcium binding protein A1

S100A1

0.31

0.41

0.40

0.71

NM_006271

S100 calcium binding protein A12

S100A12

10.98

0

5.13

1.09

FJ263393.1

S100 calcium binding protein A8

S100A8

12.96

0

6.83

0

FJ263391.1

S100 calcium binding protein A9

S100A9

20.00

0

9.71

0

FJ263392.1

serum amyloid A1

SAA1

14.59

0.54

1.82

24.35

NM_199161

secreted phosphoprotein 1

SPP1

2.66

2.39

1.67

24.35

AK296035

tumor necrosis factor, alpha-induced protein 6

TNFAIP6

6.54

0

3.26

0.58

NM_001159607

Lipid biosynthesis

Lysophosphatidic acid acyltransferase, delta

AGPAT4

2.34

0

1.48

0.58

NM_020133

lysophosphatidic acid acyltransferase, zeta

AGPAT6

1.56

0.54

2.03

0

NM_178819

alkylglycerone phosphate synthase

AGPS

2.41

0.95

1.46

32.28

NM_003659

farnesyl-diphosphate farnesyltransferase 1

FDFT1

0.77

14.04

0.45

1.82

AK297868

isopentenyl-diphosphate delta isomerase 1

IDI1

0.84

26.53

0.46

2.88

NM_004508

inositol-3-phosphate synthase 1

ISYNA1

2.12

0.54

1.65

1.44

NM_016368

stearoyl-CoA desaturase

SCD

2.46

0.54

3.37

8.49

AB208982

triosephosphate isomerase 1

TPI1

3.00

0

2.11

3.33

NM_000365

Locomotion

CD97 molecule

CD97

1.50

3.06

2.17

1.64

NM_001784

coronin, actin binding protein, 1A

CORO1A

2.64

0.54

1.26

4.65

NM_007074

chemokine ligand 10

CXCL10

7.69

4.93

2.58

1.07

NM_001565

Kallmann syndrome 1 sequence

KAL1

0.43

1.44

0.46

4.65

NM_000216

mitogen-activated protein kinase 1

MAP2K1

2.03

0

1.17

1.7

NM_002755

platelet factor 4

PF4

4.15

0

3.80

1.64

NM_002619

phosphatidic acid phosphatase type 2B

PPAP2B

8.45

0.95

3.04

11.46

NM_003713

prostaglandin-endoperoxide synthase 2

PTGS2

5.75

0

5.41

0

NM_001784

Lyase activity

aldolase C, fructose-bisphosphate

ALDOC

3.08

0

2.18

3.33

AK294157

carbonic anhydrase XIII

CA13

0.41

0.82

0.40

0.71

NM_198584

enolase 1, (alpha)

ENO1

2.24

0

1.80

4.94

AK298600

N-acetylneuraminate pyruvate lyase

NPL

1.71

1.62

2.28

0

AK297017

serine dehydratase

SDS

7.58

0

9.14

0

NM_006843

Antigen processing and presentation

Major histocompatibility complex, class II, DO alpha

DOA

2.29

0

1.42

13.11

NM_002119.3

Major histocompatibility complex, class II, DO beta

DOB

17.85

2.39

3.78

1.64

NM_002120.3

Major histocompatibility complex, class II, DQ alpha 1

DQA1

2.07

1.62

1.37

9.21

M33906.1

Major histocompatibility complex, class II, DQ beta 1

DQB1

2.47

0

2.14

11.46

M32577

Major histocompatibility complex, class II, DR alpha

DRA

7.87

0

3.69

1.08

NM_001134339

Major histocompatibility complex, class II, DR beta 1

DRB1

5.83

0.95

2.17

1.17

AY770514.1

Signal transducer activity

guanine nucleotide binding protein (G protein), gamma 2

GNG2

1.94

0.41

2.26

0.42

NM_0530

regulator of G-protein signaling 1

RGS1

1.02

58.23

0.47

4.25

NM_002922

transforming growth factor, alpha

TGFA

2.79

0

1.54

14.86

AK312899

wingless-type MMTV integration site family, member 6

WNT6

0.49

1.09

0.64

21.46

NM_006522

Antiviral immune response

chemokine ligand 8

CCL8

8.02

0

3.17

0

NM_214214.1

extracellular matrix protein 1

ECM1

2.01

0.54

2.89

0

CB477333

IFN-gamma

IFNG

0.49

0.41

3.18

0

NM_213948.1

interleukin 18

IL18

2.58

0.95

5.84

19.25

NM_001562

ISG15 ubiquitin-like modifier

ISG15

1.56

10.92

2.51

0

EU584557.1

interferon-stimulated gene 20 kDa protein

ISG20

1.49

61.10

2.20

4.94

NM_002201

vasoactive intestinal peptide

VIP

0.69

32.11

4.14

0

NM_194435

Apoptosis

BCL2/adenovirus E1B 19kDa interacting protein 3

BNIP3

3.69

0

2.61

2.88

U15174.1

CD14 molecule

CD14

3.60

0

3.87

0

NM_001097445

immediate early response 3

IER3

5.46

0.41

2.21

1.64

NM_003897

lectin, galactoside-binding, soluble, 1

LGALS1

0.42

0.82

0.66

10.51

NM_002305

interleukin 1 alpha

IL1A

21.31

0

8.47

0

NM_214029.1

interleukin 1, beta

IL1B

18.69

0

10.33

2.88

M15330.1

serine/threonine kinase 17a

STK17A

1.53

1.62

2

1.64

NM_00476

tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain

TNFRSF10D

2.26

0

2.21

61.11

NM_003840

tumor necrosis factor alpha

TNFα

5.13

0.41

1.96

6.28

EU682384.1

Cell proliferation

Betacellulin

BTC

2.26

0.95

1.78

1.97

NM_001729

epithelial membrane protein 1

EMP1

0.49

0.82

0.62

2.88

NM_001423

fatty acid binding protein 3, muscle and heart

FABP3

1.88

0.95

2.57

3.09

NM_004102

fms-related tyrosine kinase 1

FLT1

1.91

1.78

2.49

0

NM_002019

interleukin 2 receptor, gamma

IL2RG

1.39

6.28

2.06

1.07

NM_000206

interleukin 8 receptor, beta

IL8RB

1.33

45.16

3.41

0

NM_001557

v-myc myelocytomatosis viral oncogene homolog

MYC

0.75

14.04

0.29

0.71

NM_002467

pro-platelet basic protein

PPBP

2.75

0.95

3.57

1.97

NM_002704