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Table 2 Priers used for qRT-PCR validation and additional expression profiling

From: Transcription analysis of the porcine alveolar macrophage response to porcine circovirus type 2

Gene

Primer sequence, 5′-3′

Amplicon length, bp

GenBank accession number

HPRT-1 a

Forward:

TCATTATGCCGAGGATTTGGA

90

DQ136030

 

Reverse:

CTCTTTCATCACATCTCGAGCAA

  

GAPDH b

Forward:

TGCCAACGTGTCGGTTGT

120

NG_007073.2

 

Reverse:

TGTCATCATATTTGGCAGGTTT

  

CD14

Forward:

AGAGTTCAAAGAGTAGGGAA

86

NM_001097445.2

 

Reverse:

AACGCTATCTGTCCTCAC

  

GPNMB

Forward:

CCCTCACGAGCACCCTTGT

91

NM_001098584

 

Reverse:

AAGAAGCCAACGAAGACCAGGAT

  

ICAM1

Forward:

GCAAGAAGATAGCCAACCA

106

NM_000201.2

 

Reverse:

TGCCAGTTCCACCCGTTC

  

IFNγ

Forward:

AAAGATAACCAGCCCATTC

93

NM_213948.1

 

Reverse:

GTCATTCAGTTTCCCAGA

  

IL6

Forward:

CCTTCAGTCCAGTCGCCTTCTCC

97

NM_214399.1

 

Reverse:

GCATCACCTTTGGCATCTTCTTCC

  

IL8

Forward:

CACTGTGAAAATTCAGAAATCATTGTTA

105

NM_213867.1

 

Reverse:

CTTCACAAATACCTGCACAACCTTC

  

ISG15

Forward:

GGAGGGTAGGGAGGAGGGA

108

EU584557.1

 

Reverse:

ATGGGCGTCACACAGGCT

  

S100A1

Forward:

GGGAGAACAGTTGAGCAGATGGT

104

NM_006271.1

 

Reverse:

GGAGGGAACAGCGGGATGAA

  

S100A12 c

Forward:

GGCATTATGACACCCTTATC

168

NM_001160272.1

 

Reverse:

GTCACCAGGACCACGAAT

  

S100A4

Forward:

GGGGAAAAGGACGGATGAAGC

96

XM_001929560

 

Reverse:

GCAGGACAGGAAGACGCAGTA

  

S100A8 c

Forward:

GCGTAGATGGCGTGGTAA

155

FJ263391.1

 

Reverse:

GCCCTGCATGTGCTTTGT

  

S100A9 c

Forward:

CCAGGATGTGGTTTATGGCTTTC

186

NM_001177906.1

 

Reverse:

CGGACCAAATGTCGCAGA

  

TLR1 b

Forward:

TGCTGGATGCTAACGGATGTC

102

NM_001031775.1

 

Reverse:

AAGTGGTTTCAATGTTGTTCAAAGTC

  

TLR2 b

Forward:

TCACTTGTCTAACTTATCATCCTCTTG

162

AB085935.1

 

Reverse:

TCAGCGAAGGTGTCATTATTGC

  

TLR3 b

Forward:

AGTAAATGAATCACCCTGCCTAGCA

112

DQ647698.1

 

Reverse:

GCCGTTGACAAAACACATAAGGACT

  

TLR4 b

Forward:

GCCATCGCTGCTAACATCATC

108

AY535422.1

 

Reverse:

CTCATACTCAAAGATACACCATCGG

  

TLR5

Forward:

CTCGCCCACCACATTA

156

FJ668383

 

Reverse:

TGAGGGTCCCAAAGAGT

  

TLR6 b

Forward:

AACCTACTGTCATAAGCCTTCATTC

95

NM_213760.1

 

Reverse:

GTCTACCACAAATTCACTTTCTTCAG

  

TLR8 b

Forward:

AAGACCACCACCAACTTAGCC

105

NM_214187.1

 

Reverse:

GACCCTCAGATTCTCATCCATCC

  

TLR9 b

Forward:

CACGACAGCCGAATAGCAC

122

AY859728.1

 

Reverse:

GGGAACAGGGAGCAGAGC

  

TNFα

Forward:

TGGTGGTGCCGACAGATGG

102

EU682384.1

 

Reverse:

GGCTGATGGTGTGAGTGAGGAA

  

VCAM1

Forward:

TCAGGGAGGACACAAAGAAGGG

135

NM_213891.1

 

Reverse:

AAACGGCAAACACCATCCAAAGT

  
  1. a from reference [21], b from reference [22], c from reference [9].
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