TY - JOUR AU - Hosomichi, Kazuyoshi AU - Jinam, Timothy A. AU - Mitsunaga, Shigeki AU - Nakaoka, Hirofumi AU - Inoue, Ituro PY - 2013 DA - 2013/05/28 TI - Phase-defined complete sequencing of the HLA genes by next-generation sequencing JO - BMC Genomics SP - 355 VL - 14 IS - 1 AB - The human leukocyte antigen (HLA) region, the 3.8-Mb segment of the human genome at 6p21, has been associated with more than 100 different diseases, mostly autoimmune diseases. Due to the complex nature of HLA genes, there are difficulties in elucidating complete HLA gene sequences especially HLA gene haplotype structures by the conventional sequencing method. We propose a novel, accurate, and cost-effective method for generating phase-defined complete sequencing of HLA genes by using indexed multiplex next generation sequencing. SN - 1471-2164 UR - https://doi.org/10.1186/1471-2164-14-355 DO - 10.1186/1471-2164-14-355 ID - Hosomichi2013 ER -