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Table 2 Sequence quality and closest HLA allele designation for heterozygous samples

From: Phase-defined complete sequencing of the HLA genes by next-generation sequencing

Sample Gene Average depth Coverage(allele 1/allele 2) Luminex genotype Closest HLA genotype
E1 A 4306.6 97.1/100 02:01/31:01 02:01:01:01/31:01:02
  C 6668.5 100/99.6 03:03/03:04 03:03:01/03:04:01:01
  B 5250.9 100/100 15:11/40:01 15:11:01 * /40:01:02
  DRB1 272.2 99.7/95.6 04:05/09:01 04:05:01/09:01:02 *
  DQB1 3322.3 100/99.5 03:03/04:01 03:03:02:02/04:01:01
  DPB1 780.8 95.5/100 02:02/05:01 02:02/05:01:01 *
E8 A 4303.7 100 24:02/24:02 24:02:01:01/24:02:01:01
  C 3904.7 100/100 12:02/14:02 12:02:02/14:02:01
  B 2829.4 99.7/100 51:01/52:01 51:01:01/52:01:01:01
  DRB1 612.3 100/100 01:01/15:02 01:01:01/15:02:01
  DQB1 3132.6 100/100 05:01/06:01 05:01:01:02/06:01:01
  DPB1 588.7 100/100 04:02/09:01 04:02:01:02/09:01
E11 A 3880.2 100/100 24:02/31:01 24:02:01:01/31:01:02
  C 3695.0 100/100 01:02/07:04 01:02:01/07:04:01
  B 4490.7 100/100 15:18/54:01 15:18:01 * /54:01:01
  DRB1 501.8 78.3/99.2 04:01/04:05 04:01:01/04:05:01
  DQB1 3751.8 100/100 03:01/04:01 03:01:01:01/04:01:01 *
  DPB1 748.2 100/100 02:01/05:01 02:01:02 * /05:01:01 *
E17 A 808.4 100/100 02:07/24:02 02:07:01/24:02:01:01
  C 1708.7 100/100 01:02/12:02 01:02:01/12:02:02
  B 2507.7 100/100 46:01/52:01 46:01:01/52:01:01:02
  DRB1 2080.3 100/100 08:03/15:02 08:03:02/15:02:01
  DQB1 1638.1 100 06:01/06:01 06:01:01/06:01:01
  DPB1 1649.6 97.9/98.4 05:01/09:01 05:01:01 * /09:01
E25 A 1042.7 100/100 02:06/24:02 02:06:01/24:02:01:01
  C 2150.0 100/100 07:02/12:02 07:02:01:03/12:02:02
  B 4871.7 100/100 07:02/52:01 07:02:01/52:01:01:02
  DRB1 460.3 100/32.8 01:01/09:01 01:01:01 * /09:01:02 *
  DQB1 1396.3 100/100 03:03/05:01 03:03:02:02/05:01:01:02 *
  DPB1 2289.0 99.6/100 02:02/04:02 02:02 * /04:02:01:01 *
E28 A 1645.3 97.2/100 26:01/33:03 26:01:01/33:03:01 *
  C 824.8 99.7 03:04/03:04 03:04:01:02/03:04:01:02
  B 2799.6 100/100 40:02/40:06 40:02:01 * /40:06:01:01
  DRB1 236.5 100 09:01/09:01 09:01:02 * /09:01:02 *
  DQB1 817.5 100 03:03/03:03 03:03:02:02/03:03:02:02
  DPB1 343.0 100 02:01/02:01 02:01:02/02:01:02
E30 A 1711.4 100/100 24:02/33:03 24:02:01:01/33:03:01 *
  C 2670.8 100/100 01:02/14:03 01:02:01 * /14:03
  B 1899.1 100/100 44:03/55:02 44:03:01/55:02:01
  DRB1 399.4 17.1/99.8 09:01/13:02 09:01:02 * /13:02:01 *
  DQB1 773.1 100/97.8 03:03/06:04 03:03:02:02/06:09 *
  DPB1 460.4 100/100 04:01/04:01 04:01:01/04:01:01
M13 A 3689.7 97.2/100 11:01/24:02 11:01:01/24:02:01:01
  C 3386.3 100/100 01:02/04:01 01:02:01/04:01:01:01
  B 3508.5 100/100 15:01/59:01 15:01:01:01/59:01:01:01
  DRB1 1029.3 100/99.5 04:05/09:01 04:05:01/09:01:02 *
  DQB1 577.6 100/100 03:03/04:01 03:03:02:02/4:01:01
  DPB1 1981.8 100/100 04:02/05:01 04:02:01:02/05:01:01 *
M14 A 1690.3 100/100 31:01/33:03 31:01:02/33:03:01
  C 4353.2 100/100 04:01/06:02 04:01:01:01/06:02:01:01
  B 4733.5 100/100 15:01/57:01 15:01:01:01/57:01:01
  DRB1 446.2 100/99.5 15:01/07:01 15:01:01:02*/07:01:01
  DQB1 763.0 98.8/98.9 03:03/06:02 03:03:02:01/06:02:01
  DPB1 1209.5 99.8/100 09:01/04:02 09:01/04:02:01:02 *
M15 A 1153.2 100/100 31:01/33:03 31:01:02/33:03:01
  C 1448.1 99.7/99.8 14:02/14:03 14:02:01/14:03
  B 3312.9 100/100 44:03/51:01 44:03:01/51:01:01
  DRB1 463.4 100 04:05/04:05 04:05:01/04:05:01
  DQB1 280.9 100 04:01/04:01 04:01:01 * /04:01:01 *
  DPB1 2932.9 99.5/100 02:01/03:01 02:01:02/03:01:01
M20 A 7631.1 100/100 02:06/11:01 02:06:01/11:01:01
  C 7675.7 100/100 03:03/08:01 03:03:01/08:01:01
  B 7566.7 100/100 15:02/35:01 15:02:01/35:01:01:01 *
  DRB1 4746.2 100 15:01/15:01 15:01:01:01/15:01:01:01
  DQB1 6952.9 100/100 06:01/06:02 06:01:01/06:02:01
  DPB1 4631.3 100/99.6 05:01/02:02 05:01:02/02:02
  1. Percentage of the HLA gene covered by reads that passed the Q20 threshold for nucleotide quality.
  2. *Mismatched bases with recorded sequences in IMGT/HLA database observed.
  3. Homozygous HLA allele.