Actinobacillus pleuropneumoniaegenes expression in biofilms cultured under static conditions and in a drip-flow apparatus

Table 1 Total volume, biomass and height of different A. pleuropneumoniae S4074biofilms as determined by CLSM and image analysis

Biofilm	Total volume (μm³)	Biomass (μm³/μm²)	Height
Biofilm	Total volume (μm³)	Biomass (μm³/μm²)	Average (μm)	Maximum (μm)
Live (SYTO-9)
Microtiter 4 h	866286 ±240194	8.58 ±2.38	3.99 ±0.44	41.06 ±1.63
Microtiter 6 h	627207 ±80602	6.21 ±0.8	4.58 ±1.26	41.43 ±2.49
Drip flow 4 h^a	199781 ±13424	1.98 ±0.13	3.71 ±0.03	40.07 ±2.04
Drip flow 28 h^b	900785208 ±51873026	89.21 ± 5.13	49 ±8.33	304.33 ±18.8
Dead (Propidium iodide)
Microtiter 4 h	620814 ±148835	6.15 ±1.47	3.43 ±0.21	38.70 ±1.43
Microtiter 6 h	540838 ±146407	5.36 ±1.45	3.53 ±0.31	40.02 ±3.7
Drip flow 4 h	241597 ±99038	2.39 ±0.98	3.42 ±0.13	40.61 ±2.84
Drip flow 28 h	742364385 ±89082908	73.52 ±8.82	39.49 ±6.91	302.47 ±20.79
Live-Dead Colocalization
Microtiter 4 h	435442 ±164297	4.31 ±1.63	4.47 ±0.44	36.10 ±1.56
Microtiter 6 h	359642 ±123216	3.56 ±1.22	4.61 ±0.94	37.66 ±3.76
Drip flow 4 h	96354 ±28557	0.95 ±0.28	2.95 ±0.11	36.98 ±4.32
Drip flow 28 h	480477866 ±93441665	47.58 ±9.25	43.29 ± 7.39	252.39 ± 23.7
WGA-Oregon Green
Microtiter 4 h	627544 ±182810	6.21 ±1.81	3.74 ±0.89	33.45 ±6.89
Microtiter 6 h	541858 ±145811	5.37 ±1.44	4.22 ±1.8	25.78 ±6.86
Drip flow 4 h	560824 ±54936	5.55 ±0.54	5.59 ±0.87	45.88 ±0.55
Drip flow 28 h	552328813 ±52239741	54.70 ± 5.17	42.01 ±3.02	273.41 ±14.03

^aThis represents the biofilm formed during the 4 h pre-incubation prior to the initiation of the flow of fresh medium.
^bImages were obtained with a 4X objective rather than a 40X objective.

ISSN: 1471-2164