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Table 1 Total volume, biomass and height of different A. pleuropneumoniae S4074biofilms as determined by CLSM and image analysis

From: Actinobacillus pleuropneumoniaegenes expression in biofilms cultured under static conditions and in a drip-flow apparatus

Biofilm Total volume (μm3) Biomass (μm3/μm2) Height
Average (μm) Maximum (μm)
Live (SYTO-9)
  Microtiter 4 h 866286 ±240194 8.58 ±2.38 3.99 ±0.44 41.06 ±1.63
  Microtiter 6 h 627207 ±80602 6.21 ±0.8 4.58 ±1.26 41.43 ±2.49
  Drip flow 4 ha 199781 ±13424 1.98 ±0.13 3.71 ±0.03 40.07 ±2.04
  Drip flow 28 hb 900785208 ±51873026 89.21 ± 5.13 49 ±8.33 304.33 ±18.8
Dead (Propidium iodide)
  Microtiter 4 h 620814 ±148835 6.15 ±1.47 3.43 ±0.21 38.70 ±1.43
  Microtiter 6 h 540838 ±146407 5.36 ±1.45 3.53 ±0.31 40.02 ±3.7
  Drip flow 4 h 241597 ±99038 2.39 ±0.98 3.42 ±0.13 40.61 ±2.84
  Drip flow 28 h 742364385 ±89082908 73.52 ±8.82 39.49 ±6.91 302.47 ±20.79
Live-Dead Colocalization
  Microtiter 4 h 435442 ±164297 4.31 ±1.63 4.47 ±0.44 36.10 ±1.56
  Microtiter 6 h 359642 ±123216 3.56 ±1.22 4.61 ±0.94 37.66 ±3.76
  Drip flow 4 h 96354 ±28557 0.95 ±0.28 2.95 ±0.11 36.98 ±4.32
  Drip flow 28 h 480477866 ±93441665 47.58 ±9.25 43.29 ± 7.39 252.39 ± 23.7
WGA-Oregon Green
  Microtiter 4 h 627544 ±182810 6.21 ±1.81 3.74 ±0.89 33.45 ±6.89
  Microtiter 6 h 541858 ±145811 5.37 ±1.44 4.22 ±1.8 25.78 ±6.86
  Drip flow 4 h 560824 ±54936 5.55 ±0.54 5.59 ±0.87 45.88 ±0.55
  Drip flow 28 h 552328813 ±52239741 54.70 ± 5.17 42.01 ±3.02 273.41 ±14.03
  1. aThis represents the biofilm formed during the 4 h pre-incubation prior to the initiation of the flow of fresh medium.
  2. bImages were obtained with a 4X objective rather than a 40X objective.