Figure 3
From: Genetic association of OPRgenes with resistance to Hessian fly in hexaploid wheat
Gene markers tested on N1AT1D, N1BT1D, and N1DT1B of CS nullisomic–tetrasomic lines. PCR products amplified for TaOPR1-A, TaLOX1-A, and TaOPR7-B. Lanes: 1 N1AT1D, 2 N1BT1D, 3 N1DT1B, 4 Jagger, 5 2174. M Molecular marker. A) TaOPR1-A marker. PCR was performed using primers OPRC1-ABD-F2 and OPRC1-R8. B) TaOPR7-B marker. PCR was performed using primers OP1-C1F1 and OP1-R1. C) TaLOX1-A marker. PCR was performed using primers LOX-C5-F5 and LOX-C5-R6. Expected size of PCR products was indicated in Table 1.