Figure 6
Specificity change of TaqII REase in the presence of SIN and DMSO. Relaxed recognition sites were determined by shotgun cloning and sequencing of TaqII restriction fragments obtained in the presence of SIN and DMSO. After digestion DNA was blunted with T4 polymerase and cloned into the SmaI site of pUC19 vector. TaqII – canonical recognition sequences. TaqII*/SIN – variants of SIN induced relaxed recognition sequences.