Figure 7

Digestion of complex bacterial genomes with various sizes and GC contents. TaqII affinity star cleaved 0.5 μg bacterial (E. coli and T. thermophilus) genomic DNA samples were electrophoresed on 1.5% agarose/TBE gel. Cleavage (maximum extend) was carried out under specificity change stimulatory conditions (see Methods Figures 2, 3 and 6). (A) TaqII affinity star cleavage of E. coli genomic DNA (51% GC, 4.6 Mb [GeneBank CP000948]). Lane M1, Fermentas 1 kb DNA Ladder (selected bands marked); lane M2, Fermentas 100 bp DNA Ladder (selected bands marked); Lane K, undigested genomic DNA; lanes 1–4, DNA digested with TaqII: lane 1, without SIN and DMSO; lane 2, with SIN added, no DMSO; lane 3, no SIN, 20% DMSO; lane 4, with SIN and 20% DMSO. (B) TaqII affinity star cleavage of T. thermophilus genomic DNA (69.4% GC, 1.89 Mb [GenBank AE017221]). Reactions were conducted as in Panel A, except that T. thermophilus genomic DNA was used as substrate. (C) TaqII affinity star cleavage of horse genomic DNA (51% GC, 2.47 Gb [GenBank AAWR02000000]). Reactions were conducted as in Panel A, except that horse genomic DNA was used as substrate.