Time-course miRNA transcriptome profile of EGF-treated HeLa cells using Agilent miRNA arrays. Agilent miRNA microarrays were used to analyze miRNA expression in HeLa cells treated with EGF at the indicated time points. Average differential miRNA expression between EGF and control was calculated and the number differentially regulated probes (FC > 1.2) was plotted for each time-course point. Different lines show numbers of probes found: up-regulated (blue and purple), down-regulated (gray and green), regulated up or downwards (yellow and brown). Blue-gray-yellow: all regulated probes, without filtering for intensity; purple- green-brown: only regulated probes when at least half of the samples had log2intensity >7 (arbitrary detection limit to consider a miRNA as not absent).