Figure 5

Growth of O. piceae on malt extract agar (MEA) treated with various volumes of mixed monoterpenes (MT). Fresh fungal mycelia were used as starting material and treated with 50, 100, 200 μl MT (equivalent to 0.35, 0.7, 1.4 g/l respectively). Colony diameters were measured daily. The growth rates were calculated as mm/day at linear stage. Results are average of 3 replicates; error bars are standard deviations. MT: (+)-limonene, (+)-3-carene, racemic α-pinene and (−)-β-pinene at a ratio of 5:3:1:1. Student t-test indicated that MT applied at 100 and 200 μl/plate inhibited fungal growth significantly, but not at 50 μl/plate ( P value cutoff 0.05).