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Table 4 Validation of uniquely expressed genes induced by bleomycin in lung tissues from both Fra-1 +/+ and Fra-1 ∆/∆ mice

From: Expression profiling of genes regulated by Fra-1/AP-1 transcription factor during bleomycin-induced pulmonary fibrosis

Affymetrix ID

Symbol

Gene title

Micro array

qRT-PCR

   

WTT vs. WTC

KOT vs. KOC

WTT vs. WTC

KOT vs. KOC

   

Fold change

Fold change

Fold change

Fold change

10540085

Fbln2

Fibulin 2

2.28

ND

2.35 ± 0.27*

ND

10531737

Hpse

Heparanase

ND

1.98

ND

1.57 ± 0.10*

10587266

Gclc

Glutamate-cysteine ligase, catalytic subunit

ND

1.70

ND

1.58 ± 0.27*

10509030

Runx3

Run related transcription factor 3

ND

2.02

ND

1.30 ± 0.14*

10359697

Xcl1

Chemokine (C motif) ligand 1

ND

2.15

ND

2.57 ± 0.48*

10531420

Cxcl11

Chemokine (C-X-C motif) ligand 11

ND

2.53

ND

4.72 ± 2.33*

10590242

Ccr8

Chemokine (C-C motif) receptor 8

ND

2.13

ND

1.58 ± 0.00NS

10574213

Ccl22

Macrophage-derived chemokine

ND

2.42

ND

1.73 ± 0.54NS

10576973

Col4a1

Collagen, type IV, alpha 1

1.96

ND

1.83 ± 1.08NS

ND

10416230

Tnfrsf10b

Tumor necrosis factor receptor superfamily, member 10b

2.03

ND

2.30 ± 1.09NS

ND

10357261

Marco

Macrophage receptor with collagenous structure

ND

2.03

3.08 ± 0.11*†

2.23 ± 0.21*

10433776

Snai2

Snail homolog 2 (Drosophila)

ND

-1.92

-2.23*†

-3.50 ± 1.95*

  1. Data are presented as mean ± SD (n=3-4). *P<0.05, PBS vs bleomycin of same genotype. NS means non significant. † Result did not agree with microarray result.