Table 1 List of genes prioritized for functional evaluation of their role in adherence

SP_0018

Conserved hypothetical protein

1.7

3.3

yes (98.4%)

no

Ratio index ≥ 1.5

SP_0024

Hypothetical protein

2.6

0.9

yes (98.1%)

no

Up-regulated in the more adherent strain

SP_0025

Hypothetical protein

2.7

1.1

yes (95.7%)

yes (TmHMM)

Up-regulated in the more adherent strain

SP_0026

Hypothetical protein

2.2

X

yes (78.8%)

yes (SP, TmHMM, AR)

Up-regulated in the more adherent strain

SP_0099

Hypothetical protein

2.2

1.2

yes (98.7%)

yes (TmHMM, AR)

Up-regulated in the more adherent strain

SP_0100

Conserved hypothetical protein

2.2

1.4

yes (99.3%)

no

Up-regulated in the more adherent strain

SP_0101

Putative transporter

1.4

1.9

yes (99.1%)

yes (SP, TmHMM, AR)

Co-opted as part of an operon

SP_0415

Enoyl-CoA hydratase

1.5

0.4

yes (99.3%)

no

Co-opted as part of an operon

SP_0416

Transcriptional regulator, marR family

1.7

X

yes (99.4%)

no

Co-opted as part of an operon

SP_0417

3-oxoacyl-(acyl-carrier-protein) synthase III

1.6

1.0

yes (99.6%)

no

Co-opted as part of an operon

SP_0418

Acyl carrier protein

1.8

0.9

yes (99.8%)

no

Ratio index ≥ 1.5

SP_0419

Enoyl-(acyl-carrier-protein) reductase

1.4

0.4

yes (99.4%)

no

Co-opted as part of an operon

SP_0420

Malonyl CoA-acyl carrier protein transacylase

1.9

0.6

yes (99.5%)

no

Co-opted as part of an operon

SP_0421

3-oxoacyl-[acyl-carrier protein] reductase

2.7

0.7

yes (99.4%)

yes (SP)

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0422

3-oxoacyl-(acyl-carrier-protein) synthase II

2.4

0.7

yes (99.8%)

yes (SP)

Up-regulated in the more adherent strain

SP_0423*

Acetyl-CoA carboxylase, bitoin carboxyl carrier protein

3.6

0.9

yes (99.2%)

no

Up-regulated in the more adherent strain

SP_0424*

Similar to hydroxymyristoyl-(acyl carrier protein) dehydratase

3.6

1.0

yes (99.3%)

no

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0425*

Acetyl-CoA carboxylase, biotin carboxylase

4.2

1.1

yes (99.9%)

no

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0426*

Acetyl-CoA carboxylase, carboxyl transferase subunit beta

5.5

1.4

yes (99.9%)

no

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0427*

Acetyl-CoA carboxylase, carboxyl transferase subunit alpha

2.5

1.6

yes (99.9%)

no

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0462*

Transcriptional regulator, putative

2.1

NA

no (90.5%)

yes (SP, TmHMM, BCE)

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0463*

Cell wall surface anchor family protein

2.6

NA

no (71.3%)

yes (SP, TmHMM, AR, LPxTG)

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0464*

Cell wall surface anchor family protein

1.9

NA

no (98.3%)

yes (SP, TmHMM, AR, LPxTG)

Ratio index ≥ 1.5

SP_0465*

Cell wall surface anchor family protein

2.1

NA

no

no

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0466*

Sortase, putative

2.0

NA

no (98.6%)

yes (TmHMM)

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0467*

Sortase, putative

2.3

NA

no (96.0%)

yes (TmHMM)

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0468*

Sortase, putative

2.2

NA

no (95.8%)

yes (SP, TmHMM, AR, LAS)

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0617

Conserved domain protein

1.5

3.6

yes (97.8%)

yes (SP, TmHMM)

Ratio index ≥ 1.5

SP_0737*

Sodium-dependent transporter

2.4

X

yes (96.3%)

yes (SP, TmHMM)

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0738*

Conserved domain protein

2.0

X

yes (99.2%)

no

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_0783*

Conserved hypothetical protein

1.5

1.6

yes (96.3%)

yes (SP, TmHMM, AR)

Ratio index ≥ 1.5

SP_0879

Hypothetical protein

2

3.9

yes (99.2%)

yes (SP, BCE, AR)

Up-regulated in both strains & Ratio index ≥ 1.5

SP_1003

Conserved domain protein

0.8

3.9

yes (73.3%)

yes (SP, BCE, AR)

Up-regulated in the less adherent strain and downregulated in more adherent strain

SP_1127

Hypothetical protein

1.4

1.6

yes (98.6%)

no

Ratio index ≥ 1.5

SP_1256

Conserved hypothetical protein

X

1.9

yes (96.7%)

yes (TmHMM)

Ratio index ≥ 1.5

SP_1270*

Alcohol dehydrogenase, zinc containing

3.1

3.6

yes (99.1%)

no

Up-regulated in both strains

SP_1294*

Crcb protein downstream of a putative chorismate mutase

2.0

1.1

yes (98.6%)

yes (SP, TmHMM)

Up-regulated in the more adherent strain

SP_1295*

Crcb protein downstream of a putative chorismate mutase

1.8

1.1

yes (98.1%)

yes (TmHMM)

Co-opted as part of an operon

SP_1600

Putative membrane protein

2.0

2.0

yes (99.2%)

yes (SP, TmHMM)

Up-regulated in both strains

SP_1601

Conserved hypothetical protein

2.0

2.0

yes (99.0%)

yes (SP, TmHMM)

Up-regulated in both strains & Ratio index ≥ 1.5

SP_1602

Required for expression of the phosphonate utilization phenotype in E. coli

2.5

2.1

yes (99.0%)

no

Up-regulated in both strains & Ratio index ≥ 1.5

SP_1758*

Glycosyl transferases in the psrP-secY2A2 pathogenicity island

2.2

X

no (86.3%)

no

Up-regulated in the more adherent strain

SP_1855*

Dehydrogenase

6.0

1.0

yes (99.7%)

no

Up-regulated in the more adherent strain

SP_1856

Transcriptional regulator, merR family

7.6

1.1

yes (99.7%)

no

Up-regulated in the more adherent strain

SP_1857

Cation efflux system protein

14.4

X

yes (98.1%)

yes (SP, TmHMM)

Up-regulated in the more adherent strain

SP_1922*

Conserved hypothetical protein

2.3

1.0

yes (99.8%)

yes (BCE)

Up-regulated in the more adherent strain & Ratio index ≥ 1.5

SP_1923

Pneumolysin

3.0

0.5

yes (99.8%)

no

Up-regulated in the more adherent strain

SP_1924

Hypothetical protein

2.6

0.4

yes (99.0%)

no

Up-regulated in the more adherent strain

SP_1925

Hypothetical protein

2.3

0.4

Yes (98.2)

no

Up-regulated in the more adherent strain