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Table 5 Overview of IR spectral parameters

From: ATR-FTIR spectroscopy reveals genomic loci regulating the tissue response in high fat diet fed BXD recombinant inbred mouse strains

   Band area limits  
Traits Epididymal adipose tissue Liver tissue Muscle tissue
AFAT A3006+A2957+A2922+A2853+A1743+A1464+A1160 A2963+A2927+A2854+A1745+A1455 A2961+A2927+A2854+A1741+A1457
  A3006 : A [2997:3017] cm-1 - -
  A2957 : A [2950:2966] cm-1 A2963 : A [2952:2968] cm-1 A2961 : A [2951:2968] cm-1
  A2922 : A [ 2905:2936] cm-1 A2927 : A [2900:2938] cm-1 A2927 : A [2900:2939] cm-1
  A2853 : A [2840:2861] cm-1 A2854 : A [2840:2861] cm-1 A2854 : A [2840:2861] cm-1
  A1743 : A [1730:1760] cm-1 A1745 : A [1737:1759] cm-1 A1741 : A [1735:1755] cm-1
  A1464 : A [1454:1474] cm-1 A1455: A [1437:1475] cm-1 A1457 : A [1450:1472] cm-1
  A1160 : A [1133:1200] cm-1 - -
ASATF A2922+A2853 A2927+A2854 A2927+A2854
  A2922 : A [2905:2936] cm-1 A2927 : A [2900:2938] cm-1 A2927 : A [2900:2939] cm-1
  A2853 : A [2840:2861] cm-1 A2854 : A [2840:2861] cm-1 A2854 : A [ 2840:2861] cm-1
AUNSATF A3006 : A [2997:3017] cm-1 _ _
ACOL A1340+A1320+A1280 A1340 A1340+A1282
  A1340 : A [1336:1345] cm-1 A1340: A [1332:1346] cm-1 A1340 : A [1334:1355] cm-1
  A1320 : A [1315:1327] cm-1 - -
  A1280 : A [1275:1285] cm-1 - A1282 : A [1275:1287] cm-1
APROT A1550+A1340+A1320+A1280 A1547+A1340 A1550+A1340+A1282
  A1550 : A [1506:1571] cm-1 A1547 : A [1500:1565] cm-1 A1550 : A [1500:1570] cm-1
  A1340 : A [1336:1345] cm-1 A1340 : A [1332:1346] cm-1 A1340 : A [1334:1355] cm-1
  A1320 : A [1315:1327] cm-1 - -
  A1280 : A [1275:1285] cm-1 - A1282 : A [1275:1287] cm-1
AGLYC - A1154+A1081+A1044 A1081+A1046
  - A1154 : A [1141:1174] cm-1 -
  - A1081 : A [1071:1091] cm-1 A1081 : A [1067:1100] cm-1
  - A1044 : A [1020:1050] cm-1 A1046: A [1030:1052] cm-1
   Band Area Ratio  
saturated to unsaturated fat ratio (A2922+A2853)/A3006 _ _
acyl chain length A2922/A2957 A2927/A2963 A2927/A2961
collagen integrity A1550/(A1340+A1320+A1280) A1547/A1340 A1550/(A1340+A1282)
lipid to protein ratio (A3006+A2957+A2922+A2853+A1743+A1464+A1160)/ (A1550+A1340+A1320+A1280) A2963+A2927+A2854+A1745+A1455/ A1547+A1340 A2961+A2927+A2854+A1741+A1457/ A1550+A1340+A1282
  1. AFAT, total fat; ASATF, saturated fat ; AUNSATF, unsaturated fat; ACOL, collagen; APROT, total protein; AGLYC, glycogen. “A” is the area obtained by “method B” integration bands in OPUS. The min-max normalized data were used for area calculation.
  2. These parameters were used for the quantitative evaluation of compositional differences in reproductive adipose tissue, liver and muscle of BXD RI strains.
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