Figure 1

Archaeal nucleosomes protect ~60 bp chromosomal DNA fragments from micrococcal nuclease (MN) digestion. (a). Ethidium bromide stained electrophoretic separation of DNA molecules protected from MN digestion of T. kodakarensis TS517 chromatin. The control lane (C) contained double-stranded DNA size standards (bp). As indicated, MN digestion generated DNA molecules that migrated to form a band consistent with ~60 bp molecules. These were isolated and sequenced. (b). The number and size profile of the sequences of the ~60 bp DNA fragment generated by ABI-SoLiD deep sequencing.