Phylogenetic analysis of MKKs and domain analysis of seven BnaMKKs. Phylogenetic relationship of BnaMKKs with MKKs from other species. The respective MKK proteins are depicted by a two to three-letter code denoting the species in combination with numbers indicating the specific MKK gene from each species. The phylogenetic tree was reconstructed using the maximum parsimony method of MEGA5.1 program. The green highlighted proteins are from canola. The analysis involved 39 amino acid sequences. There are a total of 594 positions in the final dataset. OtMKK, an MKK from the marine green alga Ostreococcus tauri (Ot) was used to root the tree. The numbers on the nodes are percentages from a bootstrap analysis of 500 replicates. At, A. thaliana; Bd, Brachypodium distachyon; Bna, Brassica napus; Cr, Chlamydomonas reinhartdii; Gh, G. hirsutum; Nt, N. tabacum; Ot, O. tauri; Os, O. sativa; Pc,P. crispum; Sl, S. lycopersicum and Zm, Z. mays.