Sequence analysis of BnaMKKs. (A) Partial amino acid alignment of the seven BnaMKK proteins. Multiple sequence alignment was performed using the ClustalX1.83 and illustrated by BOXSHADE (http://www.ch.embnet.org/software/BOX_form.html). Identical amino acids are shaded in black, and similar amino acids are shaded in gray. The docking and phosphorylation sites are indicated by overbars, with the lysine (K) necessary for the kinase activity is boxed. (B) Schematic diagram of amino acid motifs of BnaMKKs. Motif analysis was determined by using MEME4.0 program as described in Methods. Different colors of the boxes represent 10 motifs in the corresponding position of each BnaMKK proteins. The information of 10 motifs was illustrated in Additional file 4: Figure S2.