Phylogenetic analysis of MPKs and domain analysis of 12 BnaMPKs. Phylogenetic relationship of BnaMPKs with MKKs from other species. The respective MPK proteins are depicted by a two to three-letter code denoting the species in combination with numbers representing the exact MKK from this species. The phylogenetic tree was reconstructed through maximum parsimony method of MEGA5.1 program. The green highlighted proteins are BnaMPKs. The analysis involved 85 amino acid sequences. There are a total of 752 positions in the final dataset. The numbers on the nodes are percentages from a bootstrap analysis of 500 replicates. The atypical MEY motif in the activation loop of a few MPKs is marked by arrows. OtMPK is used as the outgroup. At, A. thaliana; Bd, Brachypodium distachyon; Bna, Brassica napus; Ca, C. annuum; Gm, G. max; Gh,G. hirsutum; Ms, M. sativa; Msi, Malus sieversii; Nt,N. tabacum; Os,O. sativa; Ot,O. tauri; Pc,P. crispum; Ps, P. sativum; Pt, P. trichocarpa; Sl, S. lycopersicum; Ta,T. aestivum and Zm, Z. mays.