Figure 2

Shared and distinct sites of occupation. Hierarchical clustering (average linkage algorithm) was performed with normalized ChIP-seq tags (tags per 10 million), which are located within +/− 1.5 kb flanking regions of the top 1000 STAT peak centers (determined by height of peaks in each condition). GAS motifs were identified within +/− 75 bp flanking regions of the top sites using the MOODS algorithm (p value < 0.01) [26]. Rows represent the top 1000 STAT binding sites in the given cell type (top label) and red vertical lines in tracks indicate the binding of STAT under specific conditions (bottom labels). (A) STAT1 peak centered in macrophages with IFNγ stimulation. (B) STAT3 peak centered in ES cells with LIF stimulation. (C) STAT5 peak centered in 3T3-L1 cells with adipogenic inducers. (D) STAT5 peak centered in T cells with IL-2 stimulation. (E) STAT5 peak centered in STAT5A overexpressing MEF with growth hormone. (F) STAT5 peak centered in female liver with growth hormone.