NFR shrinkage and DS nucleosome dynamics in rsc4-∆4 mutant. A) Nucleosome heat maps of 120 genes showing nucleosome changes in rsc4-∆4 mutant as compared to the wild type cells. B) Real Time ChIP-PCR validation of a nucleosome encroaching the otherwise NFR, tA(AGC)D gene body. C) Comparison of the averaged nucleosome occupancy for 120 genes shown in the panel A. D) RPC160-myc (pol III) occupancy does not differ significantly in the two strains. E) RSC (Sth1-myc) occupancy shows somewhat higher levels in the mutant.